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pE4
pE4
規(guī)格:
價格:
編號:B213619
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pE4
商品貨號
B213619
Designations
pE4
GenBank Number

M10988

X51416

培養(yǎng)基
Species
Homo sapiens, human
Depositors
Cetus Corp., A Belt, Cetus Corp.
Vector
Construct size (kb): 4.80
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references:
Insert
DNA: cDNA
Genome: human
Gene symbol: TNFA
Genomic copy number: unique
Gene name: tumor necrosis factor, alpha (cachectin)
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 p21.3
Tissue: HL-60 cell line (promyelocytic leukemia)
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 1.585
Cross references: DNA Seq. Acc.: M10988
Insert lengths(kb): 1.59
Tissue: HL-60 cell line (promyelocytic leukemia)
Gene product: tumor necrosis factor, alpha (cachectin) [TNFA]
Insert Size (kb)
1.585
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains recognition sites for the following enzymes: HgiA1 (2), BglI (2), NarI, AvaI, PvuII (2), HindIII, EcoRI, PstI, StuI (2).
A PstI digest will release a 1.1 kb fragment which contains the TNF coding sequence plus some vector sequence at the 5' end and some 3' untranslated sequence.
Restriction digests of the clone give the following sizes (kb): PstI--2.4, 1.4, 1.1; HindIII--3.4, 1.5; BamHI--3.1, 1.8; EcoRI--4.6; EcoRI/PstI--2.4, 1.5, 1.0; EcoRI/BamHI--3.1, 1.1, 0.7; HindIII/PstI--2.4, 0.9, 0.8, 0.5;
BamHI/HindIII--2.7, 1.0, 0.74, 0.47; StuI--3.5, 2.2, 0.25.
References

Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,063 dated Jun 30 1987

Mark DF, et al. Human tumor necrosis factor. US Patent 4,677,064 dated Jun 30 1987

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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