This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level
1
Host
Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 9.8999996185302730
Vector: pKBY2 (cosmid)
Construction: pBR329, Aspergillus nidulans trpC, pJB8 cos
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
This is a cosmid vector for isolating genes by complementation of mutations in Aspergillus nidulans. It can also be used for library construction.
A 2.1 kb HindIII/SalI fragment (pJB8) containing lambda cos was ligated to HindIII/SalI-digested pBR329. A 4.1 kb XhoI fragment with wild-type Aspergillus nidulans trpC was ligated into the SalI, and a BamHI linker into the NruI site of pBR329.
The BamHI site was removed from trpC by sodium bisulfite mutagenesis.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References
Yelton MM, et al. A cosmid for selecting genes by complementation in Aspergillus nidulans: selection of the developmentally regulated yA locus. Proc. Natl. Acad. Sci. USA 82: 834-838, 1985.
