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pBC-KC
pBC-KC
規(guī)格:
價(jià)格:
編號(hào):B213669
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pBC-KC
商品貨號(hào)
B213669
Designations
pBC-KC
GenBank Number

J04596

培養(yǎng)基
Species
Mus musculus, mouse
Depositors
CD Stiles
Applications
It is frequently used as a molecular marker for response to cellular growth factors.
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: cDNA
Insert lengths(kb): 0.800000011920929
Tissue: 3T3 (clone A31) cell line
Gene product: GRO1 oncogene (melanoma growth stimulating activity, alpha)(growth regulated (alpha), growth regulated (group I)) [Mgsa]
Insert Size (kb)
0.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.8, 0.84; EcoRI--3.85; HindIII--3.85.
KC is one of several competence genes (including JB, JE) that are induced with the addition of platelet-derived growth factor. It is frequently used as a molecular marker for response to cellular growth factors.
Detects a mRNA of 1.2 kb after PDGF induction. The level of induction is increased by cycloheximide treatment.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Cochran BH, et al. Molecular cloning of gene sequences regulated by platelet-derived growth factor. Cell 33: 939-947, 1983. PubMed: 6872001

Sakaguchi AY, et al. Mouse melanoma growth stimulator activity gene (Mgsa) is polymorphic and syntenic with the W, patch, rumpwhite, and recessive spotting loci on chromosome 5. Genomics 5: 629-632, 1989. PubMed: 2575589

Oquendo P, et al. The platelet-derived growth factor-inducible KC gene encodes a secretory protein related to platelet alpha-granule proteins. J. Biol. Chem. 264: 4133-4137, 1989. PubMed: 2917992

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