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當(dāng)前位置: 首頁 > ATCC代理 > cHuVim1 [cHuVIM1] B213704
cHuVim1 [cHuVIM1]
cHuVim1 [cHuVIM1]
規(guī)格:
價(jià)格:
編號(hào):B213704
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
cHuVim1 [cHuVIM1]
商品貨號(hào)
B213704
Designations
cHuVim1 [cHuVIM1]
Species
Homo sapiens, human
培養(yǎng)基
Applications
Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences.
cHuVim1 has been used to detect a 2.0 kb mRNA in various mouse cell lines.
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: cDNA
Insert lengths(kb): 1.100000023841858
Tissue: fibroblast
Gene product: vimentin [VIM]
Alleles: A1, A2
Insert Size (kb)
1.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Insert contains the following sites separated by (bp) (approx): EcoRI- 130- MspI- 120- MspI- 35- HincII- 95- PstI- 115- PstI- 100- MspI- 140- PstI- 140- MspI- 170- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.2; HindIII--4.0; PstI--3.1, 0.46, 0.29; BamHI--3.8; SalI--4.0.
Clone starts at nt 331 (corresponding to amino acid 111) after the ATG and extends to the stop codon. Includes part of exon 1 through exon 9.
Shows 90% homology with coding region of hamster vimentin. Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences.
cHuVim1 has been used to detect a 2.0 kb mRNA in various mouse cell lines.
References

Perreau J, et al. Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells. Gene 62: 7-16, 1988. PubMed: 3371665

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