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vW-8 [pvW]

規(guī)格:

價格:

編號:B213768

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

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產(chǎn)品名稱

vW-8 [pvW]

商品貨號

B213768

Designations

vW-8 [pvW]

Species

Homo sapiens, human

培養(yǎng)基

Depositors

DC Lynch

Applications

There seems to be a partial pseudogene for VWF detected by sequences from about 2.5-4.5 kb of the cDNA. For unique sequence probes from the 5' end, EcoRI/HindIII or EcoRI/BglII or EcoRI/XmaIII fragments are useful.

For unique sequence probes from the 3' end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful.

Vector

Construct size (kb): 12.0

Insert

DNA: cDNA

Insert lengths(kb): 8.800000190734863

Tissue: endothelium

Gene product: von Willebrand factor pseudogene [VWF]

Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2, , , , A1, A2, A1, A1, A2, A2

Insert Size (kb)

8.800

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--9.4, 2.9; BamHI--5.8, 5.8; HindIII--6.6, 5.3; PstI--3.4, 1.8, 1.1, 0.8, + others;

purified DNA: HindIII--6.64, 5.7; SacI--6.6, 3.8, 2.0; BamHI--6.2 doublet; EcoRI--14; EcoRI/SacI--3.8, 3.4, 3.0. 1.0.

The insert contains the following singly cutting sites: BamHI, EcoRV, HgaI, HindIII, MspI, NaeI, PvuI, RshI, Tth111II, XbaI, XmaIII, XmnI, XorII.

The following enzymes do not cut vW-8: AhaIII, AsuII, BclI, BssHII, HpaI, MluI, NdeI, NotI, NruI, SalI, SfiI, SnaBI.

This is essentially a full-length cDNA clone except for about 150 bp 5' untranslated and 100 bp 3' untranslated sequence.

For unique sequence probes from the 3' end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful. Sequences at the 3' end show homology to vole, rat, hamster, monkey, and mouse but not quail.

Codominant segregation for the TaqI polymorphism has been demonstrated in 4 families.

The BamHI polymorphism is detected with a 1.9 kb SacI fragment of the cDNA.

The EcoRI polymorphism is detected by a 3.6 kb XmnI/SalI fragment of this clone.

Enzyme(s) not detecting polymorphism (for the 1.9 kb SacI fragment probe): AvaI, BglII, BstEII, DraI, EcoRI, HindIII, KpnI, PstI (tested in 13-15 unrelated Caucasians).

Enzyme(s) not detecting polymorphism: BglII, EcoRI, SacI, TaqI.

References

Marchetti G, et al. Two additional TaqI RFLPs in von Willebrand factor gene (VWF) and pseudogene. Nucleic Acids Res. 17: 3329, 1989. PubMed: 2566970

Ewerhardt B, et al. An EcoRI polymorphism in the human von Willebrand factor (vWF) gene. Nucleic Acids Res. 17: 5416, 1989. PubMed: 2569713

Driscoll CM, et al. A KpnI DNA polymorphism in the human von Willebrand factor (VWF) gene. Nucleic Acids Res. 18: 4968, 1990. PubMed: 1975687

Bernardi F, et al. Two TaqI RFLPs in the human von Willebrand factor gene. Nucleic Acids Res. 15: 1347, 1987. PubMed: 2881274

Nishino K, Lynch DC. A polymorphism of the human von Willebrand factor (vWF) gene with BamHI. Nucleic Acids Res. 14: 4697, 1986. PubMed: 3012477

Collins CJ, et al. Molecular cloning of the human gene for von Willebrand factor and identification of the transcription initiation site. Proc. Natl. Acad. Sci. USA 84: 4393-4397, 1987. PubMed: 3496594

Bonthron D, et al. Nucleotide sequence of pre-pro-von Willebrand factor cDNA. Nucleic Acids Res. 14: 7125-7127, 1986. PubMed: 3489923

B240660：vW-8 [pvW]

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