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pHPT6
pHPT6
規(guī)格:
價(jià)格:
編號(hào):B213885
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHPT6
商品貨號(hào)
B213885
Designations
pHPT6
Species
Mus musculus, mouse
培養(yǎng)基
Depositors
C Caskey
Vector
Construct size (kb): 5.75
Insert
DNA: genomic
Insert lengths(kb): 1.389999985694885
Tissue: myeloma
Gene product: hypoxanthine phosphoribosyltransferase [Hprt]
Insert Size (kb)
1.390
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Depositor indicates the following restriction digests (kb): EcoRI--4.3, 1.39; EcoRI+BglII--4.3, 1.18, 0.207; AvaI--2.5, 3.25; AccI--3.12, 1.6, 1.07; AvaII--1.75 (doublet), 1.07, several<0.3; EcoRI+AvaII--1.75, 0.96, 0.8, 0.63, 0.4, several smaller.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 1.39; HindIII--6.2.
The insert is a subclone of lambdaHPT23 (ATCC 37598) and contains the promoter and exon 1 of the gene (105 bp of 5' untranslated sequence plus sequence coding for the first 9 amino acids).
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Melton DW, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc. Natl. Acad. Sci. USA 81: 2147-2151, 1984. PubMed: 6326107

C. Thomas Caskey, personal communication

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