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pDP122B
pDP122B
規(guī)格:
價(jià)格:
編號(hào):B213965
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
pDP122B
商品貨號(hào)
B213965
Designations
pDP122B
Species
Influenza A virus
培養(yǎng)基
Depositors
Health Research, Inc., D Panicali, Health Research, Inc.
Vector
Construct size (kb): 13.30000019073486
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR325
Intact vector size: 5.996
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmlR, tetR
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: influenza virus
Gene name: hemagglutinin
Contains complete coding sequence?: U
Strain: A/PR/8/34
Type of DNA: cDNA
Insert end: PstI
Insert end: PstI
Insert size (kb): 7.3
Cross references:
Insert lengths(kb): 7.300000190734863
Gene product: hemagglutinin
Insert Size (kb)
7.300
Media
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--5.3, 4.1, 3.75; EcoRI--7.7, 3.9, 2.45; BamHI--12.0, 1.55.
The order of fragments is as follows: PstI-BamHI (vaccinia)--2 kb; BamHI-BamHI (influenza)--1.78 kb; BamHI-PstI (vaccinia)--3.5 kb. The 2.0 kb BamHI fragment also contains an AvaI site and an EcoRI site.
This was constructed by inserting a 5.5 kb PstI fragment from vaccinia into pBR325 (modified to remove the BamHI site). A 1.8 kb HindIII fragment encoding hemagglutinin was linkered with BamHI and inserted into a BamHI site of the vaccinia insert.
Classification
Orthomyxoviridae, Influenzavirus A
References

Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988

Dennis Panicali, personal communication

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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