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pJJ281 [pJJ248]
pJJ281 [pJJ248]
規格:
價格:
編號:B213991
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pJJ281 [pJJ248]
商品貨號
B213991
Designations
pJJ281 [pJJ248]
Species
Saccharomyces cerevisiae Meyen ex E.C. Hansen
培養基
Depositors
L Prakash
Vector
Construct size (kb): 3.700000047683716
Insert
DNA: genomic
Insert lengths(kb): 0.9359999895095825
Gene product: phosphoribosylanthranilate isomerase [TRP1]
Insert Size (kb)
0.936
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--2.7, 0.7, 0.4; PvuII--2.4, 1.3; EcoRI--3.7.
A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions.
Constructed by filling in the ends of both the vector and the insert fragment.
TRP1 is in the same orientation as lacZ'.
The insert was subcloned from YRp17.
Classification
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072

Louise Prakash, personal communication

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