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pVY3

規格:

價格:

編號:B214000

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pVY3

商品貨號

B214000

Designations

pVY3

GenBank Number

U08629

培養基

Species

Yamadazyma stipitis (Pignal) Billon-Grand, teleomorph

Depositors

TW Jeffries

Vector

Construct size (kb): 4.407999992370606

Insert

DNA: genomic

Insert lengths(kb): 1.445999979972839

Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3]

Insert Size (kb)

1.446

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): SalI--4.8; SalI/XbaI--3.2, 1.6; XbaI--4.8.

The insert contains the following restriction sites (approximate kb from the 5' end): AccI--0.00, 0.62, 0.78; HindIII--0.16; KpnI--0.51; EcoRI--1.20.

References

Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063

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