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pGIBS-LYS phagemid in <em>E. coli</em>
pGIBS-LYS phagemid in <em>E. coli</em>
規格:
價格:
編號:B214022
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGIBS-LYS phagemid in E. coli
商品貨號
B214022
Designations
pGIBS-LYS phagemid in E. coli
GenBank Number

X17013

培養基
Species
Bacillus subtilis (Ehrenberg) Cohn
Depositors
M Follettie
Applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct information:
Total size of construct (kb): 3.9
Marker: ampR
Promoter giving the sense strand:T3
Promoter giving the antisense strand: T7
Excise insert: XhoI+NotI

Vector information:
Vector: pBluescriptII KS-
Vector type: phagemid
Vector size (kb): 2.961
Markers: ampR
Vector end (5’): XhoI
Vector end (3’): NotI
Insert
Insert size (kb): 1.0
Gene: lysA, diaminopimelate decarboxylase
DNA: genomic DNA
Source: Bacillus subtilis
Genbank accession: X17013 (Nucleotides 1-1016 of the insesrt correspond to nucleotides 329-1345 of X17013.)
Insert end (5’): XhoI linker
Insert end (3’): BamHI/polyA/NotI linker
Insert Size (kb)
1.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Freeze dried E. coli containing the plasmid
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 329-1345 of Genbank X17013.
An artificial polyA tail was added onto the 3' end of the insert and can be excised using BamHI + NotI. XhoI + BamHI will excise insert without the polyA tail.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.16; PstI--0.18; ClaI--0.47; BglI--0.54.
Restriction digests of the clone give the following sizes (kb): NotI--4.0; NotI/XhoI--3.0, 1.1; XhoI--4.0.
References

M Follettie, personal communication

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