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pCM190

規格:

價格:

編號:B214030

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

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產品名稱

pCM190

商品貨號

B214030

Designations

pCM190

Depositors

E Herrero

培養基

Biosafety Level

Vector Information

Size (kb): 8.0120000839233400

Vector: pCM190 (plasmid)

Promoters: Promoter CMV

Marker(s):URA3,ampR

Construct size (kb): 8.01200008392334

Features: marker(s): URA3
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: 2 micron
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1

Applications

YE-type (episomal) shuttle vector

expression vector

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--8.0; PstI--8.0; BamHI/XhoI--7.2, 0.7.

Yeast episomal expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672