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pLES2
pLES2
規格:
價格:
編號:B214037
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pLES2
商品貨號
B214037
Designations
pLES2
Depositors
DC Stein
培養基
Biosafety Level
1
Vector Information
Size (kb): 6.0000000000000000
Vector: pLES2 (plasmid)
Construction: pGC3, pUC9 lacZ
Marker(s):ampR
Construct size (kb): 6.0
Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1, pSY1005
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--6.0; HindIII--2.4, 3.6; EcoRI--6.0; AvaI--4.0, 2.1.
Contrary to the diagram in the reference, there are two AvaI sites on the vector.
The beta-lactamase production phenotype functions in both Escherichia coli and N. gonorrhoeae.
An Escherichia coli/Neisseria gonorrhoeae shuttle vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879

Stein DC, et al. Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae. Gene 25: 241-247, 1983. PubMed: 6319238

Shipped
freeze-dried
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