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Human rhinovirus 49
Human rhinovirus 49
規格:
價格:
編號:B214234
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Human rhinovirus 49
商品貨號
B214234
Classification
Picornaviridae, Rhinovirus
Agent
Human rhinovirus 49
培養基
Strain
8213
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1).
Product Format
frozen
Storage Conditions
-70°C or colder
Genome Sequenced Strain

Yes

Comments
This product is a mycoplasma free preparation derived from NIAID Research Reference Reagent V-116-002-021 (ATCC VR-1159). It replaces ATCC VR-1159 and ATCC VR-516, which will be discontinued when inventory is depleted. Strain 8213 is the prototype strain for human rhinovirus 49 and it has been fully sequenced (GenBank: DQ473496.1).
Effect on Host
Typical rhinovirus CPE in cell culture, including rounding and sloughing of infected cells.
Growth Medium
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host:H1-HeLa (ATCC CRL-1958)

Production Host: H1-HeLa (ATCC? CRL-1958™)

Alternate Hosts:WI-38 (ATCC CCL-75 ), HeLa (ATCC CCL-2 ), KB (ATCC CCL-17 ) and other human cells.
Growth Conditions
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.0°C in a humidified, 5% CO2 atmosphere for 2-7 days with rocking, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired. Freeze preparation as rapidly as possible.
Name of Depositor
NIAID, ATCC
Source
Presumed from human throat washings; derived from ATCC VR-1159 following treatment to remove mycoplasma contamination.
References

Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.

Kistler AL, et al. Genome-wide diversity and selective pressure in the human rhinovirus. Virol. J. 4: 40, 2007. PubMed: 17477878

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