Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
When paired with ATCC CRL-2899, ATCC CRL-2900, ATCC CRL-2901 or ATCC CRL-2902, this cell line can be used as a control cell line to study BCL2 function and its signaling pathway.
Neomycin resistance gene expressed
The Neo Jurkat cell line was derived by transfecting human Jurkat T cells with the empty pSFFV mammalian expression vector carrying a neomycin-resistant gene. Stable neomycin (Neo)-resistant single-cell-originated clones were isolated and expanded by selection in medium containing 1 mg/mL G418 for 14 days.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium. An inoculum of 2 x 105 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is 2 x 106cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended.Yamamoto K, et al. BCL-2 is Phosphorylated and Inactivated by an ASK1/Jun N-Terminal Protein Kinase Pathway normally Activated at G2/M. Mol Cell Biol. Dec;19(12):8469-78. 1999. PubMed: 10567572
