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pBR322-C-A3 [pC-AC]
pBR322-C-A3 [pC-AC]
規格:
價格:
編號:B214320
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBR322-C-A3 [pC-AC]
商品貨號
B214320
Designations
pBR322-C-A3 [pC-AC]
GenBank Number

M26602

培養基
Species
Homo sapiens, human
Depositors
Univ. of Texas--M.D. Anderson Cancer Center, WM Mars, GF Saunders, Univ. of Texas--M.D. Anderson Cancer Center
Applications
Can be used in Northern blots to detect mRNA abundant in chronic myelogenous leukemia but not in normal leukocytes.
Vector
Construct size (kb): 4.630000114440918
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: DEF1
Other gene symbols: MRS
Genomic copy number: unique
Gene name: defensin 1
Contains complete coding sequence?: U
Chromosome: 8; Localization: 8 p23-p22
Tissue: chronic myelogenous leukemia, Philadelphia +
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 0.33
Cross references: DNA Seq. Acc.: M26602
Insert lengths(kb): 0.3300000131130219
Tissue: chronic myelogenous leukemia, Philadelphia +
Gene product: defensin 1 [MRS]
Insert Size (kb)
0.330
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following restriction sites (with nucleotide positions according to the published sequence): PstI--186 (close to 5' end of the insert), HindIII--246, SstI--403, RsaI--448, 493.
Restriction digests of the clone give the following sizes (kb): PstI--4.6; EcoRI--4.6; PvuII--4.6; PstI/EcoRI--3.7, 0.9; PstI/PvuII--3.4, 1.4.
The insert cannot be excised by PstI alone due to a deletion in the vector. Approximately 260 bp can be cut out using a PstI/RsaI double digest.
Can be used in Northern blots to detect mRNA abundant in chronic myelogenous leukemia but not in normal leukocytes.
References

Saunders GF, Mars WM. Probe for detection of specific human leukemias. US Patent 4,857,466 dated Aug 15 1989

Mars WM, et al. A myeloid-related sequence that localizes to human chromosome 8q21.1-22. Blood 71: 1713-1719, 1988. PubMed: 3370315

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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