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HFBCG24

規(guī)格:

價(jià)格:

編號(hào):B214330

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

HFBCG24

商品貨號(hào)

B214330

Designations

HFBCG24

GenBank Number

M77976

T04940

培養(yǎng)基

Species

Homo sapiens, human

Vector

Construct size (kb): 5.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ZNF41
Gene name: zinc finger protein 41
Contains complete coding sequence?: U
Chromosome: X; Localization: X p11.3-p11.23
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Genome: Homo sapiens
Gene symbol: DXS1004E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: X; Localization: X p11.3-p11.23
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.35
Cross references: DNA Seq. Acc.: M77976; EST: EST01560; Primer: M13 Forward
DNA Seq. Acc.: T04940; EST: EST02828; Primer: M13 Reverse;
THC id: THC100628

Insert lengths(kb): 2.349999904632568

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: DNA Segment, single copy, expressed [ZNF41]

Insert Size (kb)

2.350

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.6, 0.5, 0.25.

EST01560 matches GenBank accession M92443 with 97% identity over a length of 130 nt.

EST02828 does not show a significant match to any nucleotide sequence record at this time. In particular, it does not match GenBank M92443 for zinc finger protein ZNF41.

EST01560 matches PIR accession S00754 with 66.98% identity and 77.34% similarity over the sequence range: 17-334.

This sequence record is for human zinc finger protein (HF.12).

References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434

Polymeropoulos MH, et al. Chromosomal distribution of 320 genes from a brain cDNA library. Nat. Genet. 4: 381-386, 1993. PubMed: 8401586

Parrish JE, Nelson DL. Regional assignment of 19 X-linked ESTs. Hum. Mol. Genet. 2: 1901-1905, 1993. PubMed: 8281154

J C Venter, personal communication

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