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pCT29-8
pCT29-8
規格:
價格:
編號:B214361
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCT29-8
商品貨號
B214361
Designations
pCT29-8
GenBank Number

X12548

培養基
Species
Homo sapiens, human
Applications
Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail.
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 2.111999988555908
Tissue: placenta
Gene product: acid phosphatase 2, lysosomal [ACP2]
Insert Size (kb)
2.112
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
PstI digestion also results in a 75 bp fragment not visible under standard conditions.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.18; BamHI--4.55, 0.58; KpnI--uncut; HindIII--5.2; PstI--3.5, 1.08, 0.64.
Insert encodes a 12 bp 5' untranslated region followed by a 1269 bp coding region.
When the insert was cloned into pSVL and transfected into COS cells, acid phosphatase activity was expressed and 40% was membrane-associated.
The EcoRI insert of pCT29-8 was inserted into pBEH and transfected into BHK cells. Studies of processing in this system suggest that the enzyme is synthesized and transported to lysosomes as a transmembrane protein with a short cytoplasmic tail.
The insert detects a 2.3 kb mRNA in human liver and a 21 kb EcoRI fragment in human DNA. It detects a 5.0 kb EcoRI fragment in mouse DNA.
References

Waheed A, et al. Human lysosomal acid phosphatase is transported as a transmembrane protein to lysosomes in tranfected baby hamster kidney cells. EMBO J. 7: 2351-2358, 1988. PubMed: 3056714

Pohlmann R, et al. Human lysosomal acid phosphatase: cloning, expression and chromosomal assignment. EMBO J. 7: 2343-2350, 1988. PubMed: 3191910

Regina Pohlmann, personal communication

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