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當前位置: 首頁 > ATCC代理 > cHuVim2 [cHuVIM2] B214400
cHuVim2 [cHuVIM2]
cHuVim2 [cHuVIM2]
規格:
價格:
編號:B214400
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cHuVim2 [cHuVIM2]
商品貨號
B214400
Designations
cHuVim2 [cHuVIM2]
Species
Homo sapiens, human
培養基
Applications
Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences.
Vector
Construct size (kb): 3.900000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.100000023841858
Tissue: fibroblast
Gene product: vimentin [VIM]
Alleles: A1, A2
Insert Size (kb)
1.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Depositor indicates digests give (kb): EcoRI--2.7, 0.9; HincII--2.7, 0.9; PstI--3, 0.4, 0.2; BamHI--3.6; EcoRI+HincII--2.7, 0.9.
Insert contains the following sites separated by (bp) (approx): EcoRI- 35- MspI- 35- HincII- 95- PstI- 115- PstI- 100- MspI- 140- PstI- 140- MspI- 335- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.85, 0.95; HincII--3.05, 0.92; EcoRI/HincII--2.95, 0.92; PstI--3.2, 0.44, 0.32, 0.21; BamHI--3.9.
Insert begins at nt 561 (corresponding to amino acid 187) after the ATG start and extends 157 nt after the stop codon.
This probe contains exon 4.
Shows 90% homology with coding region of hamster vimentin. Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences.
References

Perreau J, et al. Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells. Gene 62: 7-16, 1988. PubMed: 3371665

Denise Paulin, personal communication

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