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ptrphs BGH 1-13
ptrphs BGH 1-13
規格:
價格:
編號:B214428
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
ptrphs BGH 1-13
商品貨號
B214428
Designations
ptrphs BGH 1-13
Species
Bos taurus, cow
培養基
Depositors
Lucky Ltd., J Cho, Lucky Ltd.
Applications
produces protein growth hormone
Vector
Construct size (kb): 3.089999914169312
Insert
DNA: Synthetic
Insert lengths(kb): 0.6000000238418579
Gene product: growth hormone [GH]
Target Gene: growth hormone
Insert Size (kb)
0.600
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): NdeI/SalI--2.3, 0.7, 0.3; HindIII/PstI--2.0, 0.8, 0.45; PvuI/SacI--2.3, 0.9.
The vector uses a polycistronic expression system designed to reduce formation of secondary structure of the mRNA.
The promoter is followed by a ribosome binding site (RBS), the N-terminal salmon growth hormone coding sequence, a second RBS, in frame stop codon and bovine growth hormone coding sequence.
References

Cho JM, et al. Method for production of bovine growth hormone using a synthetic gene. US Patent 5,366,876 dated Nov 22 1994

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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