vector permitting visual detection of activity in colonies
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--12.5; EcoRI--9.0, 2.3, 1.2, 0.6, 0.25; PstI/BglII--10.0, 2.4.
Not self transmissible, but mobilizable by the transfer functions of RK2.
Was tested in Pseudomonas aeruginosa PAO1161, Acinetobacter calcoaceticus BD413, Rhizobium meliloti 102F34nal, and Agrobacterium tumefaciens A18.
Promoter-cloning vector permitting visual detection of activity using the promoterless luciferase gene as the reporter.
The order of the major features in this plasmid is: rpoC rho-independent transcription terminator - EcoRI/MCS/PstI - translation terminator - luc - BglII - RSF1010 - tetR.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References
Greener A, et al. Promoters of the broad host range plasmid RK2: analysis of transcription (initiation) in five species of gram-negative bacteria. Genetics 130: 27-36, 1992. PubMed: 1732166
