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XLIMPV1B
XLIMPV1B
規(guī)格:
價(jià)格:
編號:B214740
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
XLIMPV1B
商品貨號
B214740
Designations
XLIMPV1B
GenBank Number

L36339

培養(yǎng)基
Species
Xenopus laevis, frog, South African clawed
Depositors
E Hartmann
Vector
Construct size (kb): 4.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.672999978065491
Tissue: ovary
Gene product: importin 60-1 variant b [IMPV1B*]
Insert Size (kb)
1.673
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--3.0, 1.7; EcoRI--4.4, 0.3; PstI--3.3, 0.7, 0.5, 0.3; PvuII--2.5, 1.6, 0.65.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.28, 0.40; PstI--0.43, 1.10, 1.36; PvuII--1.26.
Excising the insert with BamHI/XhoI will add approximately 20 bp from the MCS of the vector sequence to the 5' end of the insert.
References

Gorlich D, et al. Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79: 767-778, 1994. PubMed: 8001116

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