黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pK18mobsacB B214844
pK18mobsacB
pK18mobsacB
規格:
價格:
編號:B214844
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pK18mobsacB
商品貨號
B214844
Designations
pK18mobsacB
Depositors
A Schaefer
培養基
Biosafety Level
1
Vector Information
Size (kb): 5.7
Vector: pK18mobsacB (plasmid)
Construction: pK18, pSUP102 (RP4 mob), sacB
Marker(s):kanR,sacB
Construct size (kb): 5.7
Features: insert detection: lacZ'
marker(s): kanR
marker(s): sacB (sucrose sensitivity)
replicon: oriT
replicon: oriV
MCS: EcoRI...HindIII
Applications
integrating vector
mobilizable vector
produces protein levansucrase
vector containing primer sites useful for sequencing
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.7; HindIII--5.7; PstI--5.7.
After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination.
Excision of the intervening plasmid sequence by a double cross-over event can be faciliated by selection on medium containing 10 percent sucrose.
The sacB gene has been modified to eliminate the HindIII and EcoRI sites in the coding region.
Differs from pK19mobsacB (ATCC 87098) only in the orientation of the polylinker.
Cloning vector allowing mobilization into a wide range of Gram- and Gram+ bacteria.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.

Schafer A, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145: 69-73, 1994. PubMed: 8045426

Shipped
frozen
Shipping Information

?Frozen E. coli containing the plasmid.

  • 王經理
    手機號:17280875617
    QQ:1438578920
  • 胡經理
    手機號:13345964880
    QQ:2438244627
  • 周經理
    手機號:17757487661
    QQ:1296385441
  • 于經理
    手機號:18067160830
    QQ:2088210172
  • 沈經理
    手機號:19548299266
    QQ:2662369050
  • 李經理
    手機號:13626845108
    QQ:972239479