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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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Charon BS(-)
Charon BS(-)
規格:
價格:
編號:B214990
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Charon BS(-)
商品貨號
B214990
Designations
Charon BS(-)
Depositors
A Swaroop
培養基
Biosafety Level
1
Vector Information
Size (kb): 43.1
Vector: Charon BS(-) (phage, lambda - replacement)
Promoters: Promoter for in vitro transcription T3
Construction: Charon 15, pBluescript KS M13(-)
Marker(s):ampR
Construct size (kb): 43.1
Features: insert detection: lacZ'
marker(s): ampR
other: left arm
other: pBluescript KS -
other: right arm
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: NotI...KpnI
MCS: SacI...NotI
Applications
expression vector
lambda vector allowing conversion to plasmid
vector containing primer sites useful for sequencing
vector for constructing cDNA libraries
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--31.0, 15.5, 6.0; EcoRI--25.0 (doublet); SalI--27.0, 13.5, 9.4; XhoI--27.0, 13.0, 9.2.
Vector for high-efficiency directional cloning. Clones can be converted to plasmids by recircularization after a NotI digest.
Charon BS(-) (ATCC 77446) and Charon BS(+) (ATCC 77447) differ in the orientation of the M13 origin of replication.
References

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

Shipped
freeze-dried
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