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CL4-1 [T-ALK]
CL4-1 [T-ALK]
規格:
價格:
編號:B215138
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CL4-1 [T-ALK]
商品貨號
B215138
Designations
CL4-1 [T-ALK]
GenBank Number

U20165

培養基
Species
Homo sapiens, human
Applications
contains sequence activin A receptor type II-like kinase
Vector
Construct size (kb): 6.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: XhoI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ACVRLK*
Gene name: activin A receptor type II-like kinase
Contains complete coding sequence?: Y
Tissue: placenta, male caucasian fetus
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EcoRI
Insert size (kb): 3.3
Cross references: DNA Seq. Acc.: D50516
Nucleotides 1-3167 of the insert correspond to
nucleotides 373-3539 of D50516 with 100% identity.
DNA Seq. Acc.: U20165
Nucleotides 1-3167 of the insert correspond to
nucleotides 1-3167 of U20165.
Insert lengths(kb): 3.299999952316284
Tissue: placenta, male caucasian fetus
Gene product: activin A receptor type II-like kinase [ACVRLK*]
Target Gene: activin A receptor type II-like kinase
Insert Size (kb)
3.300
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.3, 3.0; PstI--3.1, 2.4, 0.8; BgII--6.3; XbaI--6.3; PvuII--2.6, 2.2, 1.5.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--2.26; HincII--2.48; NcoI--0.08, 3.12; PstI--0.05, 0.84; PvuII--2.00; StuI--2.84; XbaI--0.63.
Insert contains a poly-T cloning artifact between the XhoI site and the 5' end of the gene.
References

Kawabata M, et al. Cloning of a novel type II serine/threonine kinase receptor through interaction with the type I transforming growth factor-beta receptor. J. Biol. Chem. 270: 5625-5630, 1995. PubMed: 7890683

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