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p36/6

規格:

價格:

編號:B215258

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

p36/6

商品貨號

B215258

Designations

p36/6

GenBank Number

M14056

培養基

Species

Bos taurus, cow

Depositors

BF Tack

Vector

Construct size (kb): 4.599999904632568

Insert

DNA: cDNA

Insert lengths(kb): 1.600000023841858

Tissue: MDBK kidney cell line

Gene product: annexin II( annexin II, calpactin I heavy chain, lipocortin II, calpactin I, heavy polypeptide (p36)) [Cal1h]

Insert Size (kb)

1.600

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Insert contains the following restriction sites (in bp from the 5' end): BclI--99; BglII--964; HincII--223; PstI--472; ScaI--1336; SmaI--1172; XhoI--1332.

Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.6; PstI--2.75, 1.25, 0.55; SmaI--4.6.

Contains a complete coding sequence, with 5' and 3' untranslated regions. The open reading frame is 1014 bp.

At the amino acid level, there are 6 amino acid changes between the bovine and human sequences.

References

Kristensen T, et al. Primary structure of bovine calpactin I heavy chain (p36), a major cellular substrate for retroviral protein-tyrosine kinases: homology with the human phospholipase A2 inhibitor lipocortin. Biochemistry 25: 4497-4503, 1986. PubMed: 2945590

Brian F Tack, personal communication

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