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pLGdelta178

規格:

價格:

編號:B215396

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pLGdelta178

商品貨號

B215396

Designations

pLGdelta178

Depositors

DJ Stillman

培養基

Biosafety Level

Vector Information

Size (kb): 10.0600004196167000

Vector: pLGdelta178 (plasmid)

Promoters: Promoter truncated CYC1 promoter (no UAS element)

Construction: pBR322

Marker(s):URA3,ampR

Construct size (kb): 10.0600004196167

Features: marker(s): URA3
marker(s): ampR
promoter: truncated CYC1 promoter (no UAS element)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ

Applications

YE-type (episomal) shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): XhoI--11.0; HindIII--8.7, 2.2; EcoRI--4.75, 3.45, 2.4.

This is one of three lacZ reporter plasmids that lack UAS promoter elements (ATCC 87509, 87510 & 87513). They differ by polylinker sites.

DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity.

The vector was constructed by Lenny Guarente.

Growth Conditions

Temperature: 37.0°C

References

Guarente L, et al. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell 36: 503-511, 1984. PubMed: 6319028