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pKLBglIM250-10
pKLBglIM250-10
規(guī)格:
價(jià)格:
編號(hào):B215434
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pKLBglIM250-10
商品貨號(hào)
B215434
Designations
pKLBglIM250-10
Species
Bacillus globigii Migula
培養(yǎng)基
Depositors
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Applications
produces protein modification methylase BglI
produces protein restriction endonuclease BglI
Vector
Construct size (kb): 3.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.235999941825867
Gene product: modification methylase BglI [bglR]
Target Gene: modification methylase BglI, restriction endonuclease BglI
Insert Size (kb)
1.236
Media
ATCC Medium 1065 (see below) plus ampicillin (100 ug/ml) plus kanamycin (50 ug/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of pKL143R100-1 give the following sizes (kb): BamH/EcoRI--4.0; EcoRI--4.0; EcoRI/HindIII--2.7, 1.3.
Restriction digests of pKLBglIM250-10 give the following sizes (kb): BamHI/EcoRI--7.2, 1.5; EcoRI--8.6; EcoRI+BamHI--4.3 (doublet). The intensity of these bands is less than those derived from pKL143R100-1.
References

Lunnen KD, Wilson GG. Method for producing the BglI restriction endonuclease and methylase. US Patent 5,366,882 dated Nov 22 1994

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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