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Hulambda4x-8
Hulambda4x-8
規(guī)格:
價格:
編號:B215476
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Hulambda4x-8
商品貨號
B215476
Designations
Hulambda4x-8
Species
Homo sapiens, human
培養(yǎng)基
Depositors
C Caskey
Vector
Construct size (kb): 62.0
Insert
DNA: genomic
Insert lengths(kb): 16.79999923706055
Tissue: lymphoblast GM1202 (49,XXXXY) cell line
Gene product: hypoxanthine phosphoribosyltransferase [HPRT]
Alleles: A1, A2, A3
Insert Size (kb)
16.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19, 13, 3.8.
Sequences at the 3' end of the insert apparently rearranged during cloning and include an internal artificial EcoRI site.
The region between bases -170 and -235 of the published sequence is highly GC rich (91%).
The 5' end of the insert is 430 bp upstream of ATG of exon 1. The insert contains exon 1, but not exon 2. The insert contains no canonical promoters or enhancers, but the region between -234 and -49 is necessary for transcription.
References

Patel PI, et al. Fine structure of the human hypoxanthine phosphoribosyltransferase gene. Mol. Cell. Biol. 6: 393-403, 1986. PubMed: 3023844

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