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pTOPO-ACS
pTOPO-ACS
規格:
價格:
編號:B215583
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pTOPO-ACS
商品貨號
B215583
Designations
pTOPO-ACS
GenBank Number

AF263614

培養基
Species
Homo sapiens, human
Depositors
JL Goldstein
Applications
contains sequence acetyl-CoA synthetase
Vector
Construct size (kb): 6.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR2.1-TOPO
Intact vector size: 3.908
Type of vector: plasmid
Cloning sites: HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV BstXI
NotI XhoI NsiI XbaI ApaI
Polylinker sites: HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV BstXI
NotI XhoI NsiI XbaI ApaI
Features (with orientation and position when available):
insert detection: lacZalpha, ->, 1-571
primer site: M13 reverse priming site, ->, 205-221
MCS: bases, ->, 234-357
promoter: T7, ->, 364-383
primer site: M13 forward (-20) priming site, ->, 391-406
primer site: M13 forward (-40) priming site, ->, 411-426
replicon: f1 origin, ->, 548-962
marker(s): kanR, ->, 1296-2090
marker(s): ampR, ->, 2108-2968
replicon: ColE1 origin, ->, 3113-3786
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: acetyl-CoA synthetase
Contains complete coding sequence?: Y
Strain: human HeLa
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references:
Insert lengths(kb): 2.099999904632568
Gene product: acetyl-CoA synthetase
Target Gene: acetyl-CoA synthetase
Insert Size (kb)
2.100
Media
1065 plus ampicillin plus kanamycin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/NotI--3.9, 2.1; EcoRI--3.8, 2.0.0.2; HindIII--4.1, 1.5,0.3.
The pTOPO-ACS contains a 2.1 kb insert that encodes the full length cDNA for human acetyl CoA synthetase (ACS) (amino acids 1-701). THe plasmid was constructed in the following manner.
Polymerase chain reaction was used to amplify the full length human ACS with twelve 5 prime untranslated region (UTR) nucleotides and 29 nucleotides of the 3 prime UTR. The PCR product was ligated directly into pCR 2.1-TOPO (Invitrogen).
References

Luong A, et al. Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins. J. Biol. Chem. 275: 26458-26466, 2000. PubMed: 10843999

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