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pRBBP1-I cDNA
pRBBP1-I cDNA
規格:
價格:
編號:B215641
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pRBBP1-I cDNA
商品貨號
B215641
Designations
pRBBP1-I cDNA
GenBank Number

S57153

培養基
Species
Homo sapiens, human
Depositors
GA Otterson
Applications
contains sequence retinoblastoma-binding protein 1, isoform I
Vector
Construct size (kb): 7.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: BamHI
Vector end: HindIII
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RBBP1
Gene name: retinoblastoma-binding protein 1, isoform I
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 4.0
Cross references: DNA Seq. Acc.: S57153
Insert lengths(kb): 4.0
Gene product: retinoblastoma-binding protein 1, isoform I [RBBP1]
Target Gene: retinoblastoma-binding protein 1, isoform I
Insert Size (kb)
4.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.2; BamHI/HindIII--4.1, 3.1; EcoRI--3.0, 2.4, 1.2, 0.48; HindIII--7.2; SacI--3.2, 3.0, 0.8.
References

Otterson GA, et al. Alternative splicing of the RBP1 gene clusters in an internal exon that encodes protential phosphorylation sites. Oncogene 8: 949-957, 1993. PubMed: 8455946

Fattaey AR, et al. Characterization of the retinoblastoma binding proteins RBP1 and RBP2. Oncogene 8: 3149-3156, 1993. PubMed: 8414517

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