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pEBA-4A8

規(guī)格:

價格:

編號:B215672

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pEBA-4A8

商品貨號

B215672

Designations

pEBA-4A8

Depositors

EW Miles

培養(yǎng)基

Biosafety Level

Vector Information

Size (kb): 11.6400003433227500

Vector: pEBA-4A8 (plasmid)

Promoters: Promoter Ptrc

Construction: pEBA-10

Marker(s):TRP1,ampR

Construct size (kb): 11.64000034332275

Features: primer site: 5'-TTGACAATTAATCATCCGGC-3'
promoter: Ptrc
terminator: rrnBT1
terminator: rrnBT2

Applications

contains sequence

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--11.6; EcoRI--5.5, 1.1 (5x); PstI--11.6; EcoRI/PstI--4.4; 1.1 (6x).

Overproduces alpha2 subunit of tryptophan synthase from Salmonella typhimurium.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046