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pJ3omega-MDR3 [3.27]
pJ3omega-MDR3 [3.27]
規格:
價格:
編號:B215709
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pJ3omega-MDR3 [3.27]
商品貨號
B215709
Designations
pJ3omega-MDR3 [3.27]
GenBank Number

M23234

培養基
Species
Homo sapiens, human
Applications
in another host, produces protein P glycoprotein 3/multiple drug resistance 3
Vector
Construct size (kb): 7.5
Insert
DNA: cDNA
Insert lengths(kb): 4.0
Tissue: liver
Gene product: P glycoprotein 3/multiple drug resistance 3 [MDR3]
Target Gene: P glycoprotein 3/multiple drug resistance 3
Insert Size (kb)
4.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.0, 3.5; BamHI--4.5, 3.0; AvaI--4.6, 2.0, 0.5, 0.4; EcoRI--3.6, 2.8, 1.2; PstI--2.9, 2.3, 0.9, 0.6, 0.5, 0.4.
The insert contains the following restriction sites (nt positions from the 5' end): BamHI--987; XhoI--1246; EcoRI--2816.
A full-length cDNA clone extending from nt -33 to +4002 relative to the translation initiation codon. The coding sequence is 3839 nt.
When transfected into BRO melanoma cells, the sequence expresses a protein recognized by monoclonal antibodies but does not confer multiple drug resistance.
Originally cloned using EcoRI linkers into the polylinker of lambdaZAP. Excised as a HindIII/XbaI fragment (sites from the polylinker) for insertion into pJ3omega.
References

van der Bliek AM, et al. Sequence of mdr3 cDNA encoding a human P-glycoprotein. Gene 71: 401-411, 1988. PubMed: 2906314

Schinkel AH, et al. Characterization of the human MDR3 P-glycoprotein and its recognition by P-glycoprotein-specific monoclonal antibodies. Cancer Res. 51: 2628-2635, 1991. PubMed: 1673638

Piet Borst, personal communication

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