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BL29-19

規格:

價格:

編號:B215712

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

BL29-19

商品貨號

B215712

Designations

BL29-19

GenBank Number

T26000

T25345

培養基

Species

Homo sapiens, human

Depositors

R Sanders

Applications

A weak signal was observed on chromosome 1.

Vector

Construct size (kb): 3.700000047683716

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D2S1864E
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 2
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: T25345; EST: EST019R; Primer: T3
DNA Seq. Acc.: T26000; EST: EST019; Primer: T7

Insert lengths(kb): 0.800000011920929

Tissue: BL29 cell line Burkitt's lymphoma

Disease: Burkitt's lymphoma

Gene product: DNA Segment, single copy, expressed [D2S1864E]

Insert Size (kb)

0.800

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; BamHI--3.75; HindIII--3.5, 0.25; KpnI--3.75; PstI--3.3, 0.4.

A weak signal was observed on chromosome 1.

References

Rhiannon Sanders, personal communication

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