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pDV2SP0.5
pDV2SP0.5
規(guī)格:
價格:
編號:B215745
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
pDV2SP0.5
商品貨號
B215745
Designations
pDV2SP0.5
GenBank Number

X15207

培養(yǎng)基
Species
Homo sapiens, human
Depositors
M Lefranc
Vector
Construct size (kb): 3.200000047683716
Insert
DNA: genomic
Insert lengths(kb): 0.5759999752044678
Tissue: Burkitt's lymphoma LY67 cell line
Gene product: T-cell receptor delta variable region V2 [TCRDV2*]
Alleles: A1, A2, A3
Insert Size (kb)
0.576
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.54; PstI/SacI--3.3; PstI--3.3; SacI--uncut; EcoRI--3.3; HindIII--3.3.
This is a 576 bp SmaI/PstI fragment containing the complete coding sequence for the germline TCRDV2 gene.
The promoter region of TCRDV2 has been sequenced.
The A1 and A3 alleles of the BamHI polymorphism for the Chinese population have a combined frequency of 0.85.
Hybridizes to a human genomic EcoRI fragment of 12.5 kb, a HindIII fragment of 4.0 kb, and an XbaI fragment of 3.6 kb.
References

Dariavach P, Lefranc MP. First genomic sequence of the human T-cell receptor delta2 gene (TRDV2). Nucleic Acids Res. 17: 4880, 1989. PubMed: 2526321

Chuchana P, et al. BamHI restriction fragment alleles of the human T-cell receptor delta (TRD) variable region V2. Nucleic Acids Res. 17: 3622, 1989. PubMed: 2566979

Dariavach P, Lefranc MP. The promoter regions of the T-cell receptor V9 gamma (TRGV9) and V2 delta (TRDV2) genes display short area repeats but no TATA box. FEBS Lett. 256: 185-191, 1989. PubMed: 2806544

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