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GST-Cip1
GST-Cip1
規格:
價格:
編號:B216128
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
GST-Cip1
商品貨號
B216128
Designations
GST-Cip1
GenBank Number

L25610

培養基
Species
Homo sapiens, human
Depositors
SJ Elledge
Applications
produces protein cyclin-dependent kinase inhibitor 1A (p21, Cip1) melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)
Vector
Construct size (kb): 6.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEX-2TKcs
Intact vector size: 5.000
Type of vector: plasmid
Vector end: XhoI
Vector end: EcoRI
Cloning sites: NdeI NcoI XhoI BamHI EcoRI
Polylinker sites: NdeI NcoI XhoI BamHI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: tac
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1A
Other gene symbols: CIP1; WAF1*
Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.2
Tissue: B lymphocyte (EBV transformed)
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EcoRI
Insert size (kb): 1.03
Cross references: DNA Seq. Acc.: L25610
Insert lengths(kb): 1.029999971389771
Tissue: B lymphocyte (EBV transformed)
Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) [CIP1]
Target Gene: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Insert Size (kb)
1.030
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.6, 1.5, 1.3, 0.55; EcoRI/XhoI--5.0, 1.1; KpnI--6.0; PstI/XbaI--3.4, 1.4, 1.15; PvuII--4.4, 1.5, 0.3.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.15, 0.62; KpnI--0.94.
Contains the complete coding sequence (nt 79-573 of the sequence record).
Met-1 of the product is linked to GST through the linker sequence SHMPMARGHEGPEVSSLWSRSWARIRRGTEALRGGA.
References

Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751

Stephen J Elledge, personal communication

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