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pSLH8
pSLH8
規格:
價格:
編號:B216133
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSLH8
商品貨號
B216133
Designations
pSLH8
Depositors
DR Woods
培養基
Biosafety Level
1
Vector Information
Size (kb): 4.0006999969482420
Vector: pSLH8 (plasmid)
Promoters: Promoter lac
Construction: pUC18, pKS3
Marker(s):ampR,indigo+
Construct size (kb): 4.000699996948242
Features: marker(s): ampR
promoter: lac
replicon: pMB1
Applications
contains sequence indole dioxygenase
produces protein indole dioxygenase
vector containing primer sites useful for sequencing
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--2.7, 1.4; EcoRI--4.1; BamHI--4.1.
The indigo pigment is intracellular and is produced on unsupplemented LB agar. Production is subject to catabolite repression by glucose, but is enhanced by tryptophan, indole, lactose, or IPTG.
A high-copy-number cloning vector permitting visual detection of recombinants using insertional inactivation of the Rhodococcus gene for indigo production. Includes priming sites useful for sequencing.
The M13 reverse sequencing primer and a 17mer primer [(5'-3') (CGAAGGCCGGTACCAGG)] complementary to the 5' end of the pigment gene (nt 849-832) can be used for sequencing cloned DNA inserts.
The order of the major features in this plasmid is: lacOP - EcoRI/MCS/SphI - indigo - lacZ' remnant - ampR - pMB1 ori - lacI.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Hart S, Woods DR. Construction of an insertional-inactivation cloning vector for Escherichia coli using a Rhodococcus gene for indigo production. J. Gen. Microbiol. 138: 205-209, 1992. PubMed: 1556550

Hart S, et al. Structure of a Rhodococcus gene encoding pigment production in Escherichia coli. J. Gen. Microbiol. 136: 1357-1363, 1990. PubMed: 2230720

Hart S, et al. Identification of indigo-related pigments produced by Escherichia coli containing a cloned Rhodococcus gene. J. Gen. Microbiol. 138: 211-216, 1992. PubMed: 1372930

Shipped
freeze-dried
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