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Feline panleukopenia virus
Feline panleukopenia virus
規格:
貨期:
編號:B216247
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Feline panleukopenia virus
商品貨號 B216247
Classification Parvoviridae, Parvovirus
Deposited As Feline panleukopenia
Agent Feline panleukopenia virus
Strain Philips-Roxane
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images VR-648 images
Comments Requires cells that are rapidly dividing to replicate
Effect on Host CPE, rounding and degeneration
Recommended Host
CRFK (ATCC CCL-94)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 60-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor FW Scott
Source Cat
References

King DA, Croghan DL. Immunofluorescence of Feline panleukopenia virus in cell culture: determination of immunological status of felines by serum neutralization. Can J Comp Med Vet Sci 29: 85-89, 1965. PubMed: 14281074

Slater EA, CJ Kucera. U.S. Patent Application Serial #24,408, Nov. 27, 1962.

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