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pMDY1 phagemid in <em>E. coli</em>
pMDY1 phagemid in <em>E. coli</em>
規(guī)格:
價(jià)格:
編號:B216266
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMDY1 phagemid in E. coli
商品貨號
B216266
Designations
pMDY1 phagemid in E. coli
GenBank Number

L00727

培養(yǎng)基
Depositors
CS Richards
Applications
Contains sequence useful for DNA diagnostics dystrophia myotonica dystrophia myotonica (includes dystrophia myotonia protein kinase), dystrophia myotonica 1
Detects triplet repeat expansions dystrophia myotonica dystrophia myotonica (includes dystrophia myotonia protein kinase), dystrophia myotonica 1
Vector
Total size (kb): 4.335
Excise insert: BamHI
Vector: pBluescript
Type of vector: Phagemid
Vector size (kb): 2.96
Insert site(s): BamHI
Markers: ampR
Insert
Insert size (kb): 1.377
Source: Human
DNA: genomic
Gene symbol: DM
Gene name: dystrophia myotonica
Genbank accession: L00727 (see above)
Contains complete coding sequence ?: N
Chromosome location: 19 q13.3
Insert ends: BamHI
Insert Size (kb)
1.377
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
In Escherichia coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.4; HindIII--4.4; SmaI--3.8, 0.46.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.01, 1.20; NarI--0.85; SmaI--0.93.
The GCT triplet expansion characteristic of myotonic muscular dystrophy can also be detected using this probe with BanI- or TaqI-digested genomic DNA.
References

Fu YH, et al. An unstable triplet repeat in a gene related to Myotonic Muscular Dystrophy. Science 255: 1256-1258, 1992. PubMed: 1546326

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