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pBB2T1 [16C8n]
pBB2T1 [16C8n]
規格:
價格:
編號:B216456
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBB2T1 [16C8n]
商品貨號
B216456
Designations
pBB2T1 [16C8n]
Species
Mus musculus, mouse
培養基
Depositors
ET Cocuzzi, DT Denhardt
Applications
in another host, produces protein tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
Vector
Construct size (kb): 10.97000026702881
Insert
DNA: cDNA
Insert lengths(kb): 0.6949999928474426
Tissue: fibroblast, embryo
Gene product: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor) [Timp]
Target Gene: tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
Insert Size (kb)
0.695
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): NheI--10.0, 0.7; EcoRV--6.2, 2.2, 1.4, 0.4; PstI--11.0; KpnI--11.0; EcoRI--11.0.
Insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.26; PstI--0.39.
Cotransfection of Sf9 insect cells with the clone and the linearized wild-type Autographa californica nuclear polyhedrosis virus (AcMNPV) (ATCC VR-1345) allows production of a recombinant virus expressing Timp and beta-galactosidase.
Transfected insect cells should be grown at 27C.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Cocuzzi ET, et al. Expression and secretion of active mouse TIMP-1 using a baculovirus expression vector. Inflammation 18: 35-43, 1994. PubMed: 8206645

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