Restriction digests of the clone give the following sizes (kb): EcoRI--19, 25.
The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in Escherichia coli RRI(pRK248), ATCC 47036.
A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817.
Allows expression of cloned cDNA in mammalian cells; can be used for library transduction.
Contains Mo-MLV LTR for transcription of cDNA and neo; ori of SV40 and pBR322.
References
Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791
