Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 35°C
Recommendations for Infection: Plate cells 24-36 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI. Centrifuge at 800 x g at room temperature for 75 minutes - 2 hours. End adsorption by adding growth medium.
Incubation: 15 days
Izzard L, et. al. Isolation of a novel Orientia species (O. chuto sp. nov.) from a patient infected in Dubai. J. Clin. Microbiol. 48(12): 4404-4409, 2010. PubMed: 20926708
