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cTBQ4
cTBQ4
規格:
價格:
編號:B217042
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
cTBQ4
商品貨號
B217042
Designations
cTBQ4
Species
Homo sapiens, human
培養基
Depositors
RL White, Y Nakamura
Applications
Codominant segregation of the MspI RFLP was observed in 45 three-generation families.
RFLPs were observed after competitive hybridization with total human DNA.
Vector
Construct size (kb): 48.79999923706055
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: DNA Segment, single copy [D10S27]
Alleles: A1, A2
Insert Size (kb)
40.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--11.0,8.4,6.0,4.2,3.6,2.4,2.0; HindIII--18.0,5.0,4.0,3.4,2.8,2.1,1.3; BglII--15.0,6.8,5.2,4.2,2.9,1.7,1.3,1.1; SalI--39.0, 2.5; EcoRI--10.0,5.5,3.2,2.6,2.45,1.9,1.75.
Codominant segregation of the MspI RFLP was observed in 45 three-generation families.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
RFLPs were observed after competitive hybridization with total human DNA.
Enzyme(s) not detecting polymorphism: TaqI, RsaI, BglII, PstI and PvuII.
References

White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325

Raymond L White, personal communication

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