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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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pAT3 [pAT3c, pATIII-2]
pAT3 [pAT3c, pATIII-2]
規格:
價格:
編號:B217167
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAT3 [pAT3c, pATIII-2]
商品貨號
B217167
Designations
pAT3 [pAT3c, pATIII-2]
Depositors
SH Orkin
培養基
Biosafety Level
1
Vector Information
Size (kb): 8.8999996185302730
Vector: pAT3 (plasmid)
Promoters: Promoter unknown promoter
Marker(s):ampR
Construct size (kb): 5.300000190734863
Features: marker(s): ampR
promoter: unknown promoter
enhancer: unknown enhancer
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.4; EcoRI--5.4; PstI--3.8, 1.6.
This clone includes the complete coding sequence, some 5' and 3' sequences.
This clone detects genomic EcoRI fragments of 11.0 and 4.1 kb.
The polymorphic site is located approximately 100 bp downstream of the 3' end of exon 4.
Because of the large sizes of the alleles if genomic DNA is digested with NheI alone, detection of the NheI polymorphism is improved with a NheI/SpeI digest.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Prochownik EV, et al. Isolation of a cDNA clone for human antithrombin III. J. Biol. Chem. 258: 8389-8394, 1983. PubMed: 6305982

Bock SC, Radziejewska E. A NheI RFLP in the human antithrombin III gene (1q23-q25) (AT3). Nucleic Acids Res. 19: 2519, 1991. PubMed: 1675011

Shipped
freeze-dried
Shipping Information
Distributed: freeze-dried
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