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5D5.11.6 [5D5]
5D5.11.6 [5D5]
規格:
價格:
編號:B217181
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
5D5.11.6 [5D5]
商品貨號
B217181
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
lymph node
培養基
Cell Type
hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
Derivation
Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597).
Genes Expressed
immunoglobulin; monoclonal antibody; against hepatocyte growth factor (HGF) receptor
Cellular Products
immunoglobulin; monoclonal antibody; against hepatocyte growth factor (HGF) receptor
Comments
Animals were immunized with c-Met-IgG fusion protein (cellular receptor for HGF). Lymph node cells were fused with P3X63Ag8U.1 mouse myeloma cells (ATCC CRL-1597).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1 kappa , IgG1; kappa light chain
Name of Depositor
Genetech, Inc.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonist antibodies and uses thereof. US Patent 5,686,292 dated Nov 11 1997

Schwall RH, Tabor KH. Nucleic acids encoding hepatocyte growth factor receptor antagonist antibodies. US Patent 5,646,036 dated Jul 8 1997

Schwall RH, Tabor KH. Hepatocyte growth factor receptor antagonists and uses thereof. US Patent 6,468,529 dated Oct 22 2002

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