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lambda27 [GC.27]

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價(jià)格:

編號(hào):B217187

品牌:Mingzhoubio

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DNA

RNA

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產(chǎn)品名稱

lambda27 [GC.27]

商品貨號(hào)

B217187

Designations

lambda27 [GC.27]

GenBank Number

L10641

培養(yǎng)基

Species

Homo sapiens, human

Vector

Construct size (kb): 49.79999923706055

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:

Insert

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 11.8
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~4900-16900 of the insert correspond to
nucleotides ~1-12000 of L10641.

Insert lengths(kb): 11.80000019073486

Tissue: fetal liver (ATCC 37333)

Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]

Insert Size (kb)

11.800

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--22.0, 10.0, 4.5, 2.5, 1.8, 1.7, 0.7, 0.6; HindIII-->23, 18, 6.7; BglII--20, 7.2, 5.8, 4.85, 3.9, 2.8, 2.15, 1.7, 0.9, 0.65, 0.36; BamHI-->23, 3.9, 0.5; SacI-->23, 8.8, 4.7, 3.9, 1

ATCC 95662/95663 and 95664/95665 overlap by 5. kb and share EcoRI fragments of the following sizes (kb): 0.7, 1.7.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI1 --2.5, 4.3, 4.9, 9.4, 10.1, 11.8; HindIII--1.1, 7.8; SacI--1.8, 3.3, 4.4.

Begins in the 5' flanking region and continues into intron 1.

Contains L1 (Kpn) repeats.

References

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

B243826：lambda27 [GC.27]

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