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當前位置: 首頁 > ATCC代理 > pCDM8-CD97 [CD97 cDNA] B217204
pCDM8-CD97 [CD97 cDNA]
pCDM8-CD97 [CD97 cDNA]
規格:
價格:
編號:B217204
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCDM8-CD97 [CD97 cDNA]
商品貨號
B217204
Designations
pCDM8-CD97 [CD97 cDNA]
GenBank Number

X84700

培養基
Species
Homo sapiens, human
Depositors
J Hamann
Applications
in another host, produces protein CD97 antigen
Vector
Construct size (kb): 7.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCDM8
Intact vector size: 4.356
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII XbaI XhoI BstXI PstI NotI
Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI
Construction: CMV, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1, 1-587
replicon: M13, <-, 588-1182
marker(s): supF, <-, 1183-1384
promoter: CMV, ->, 1516-2170
promoter for in vitro transcription: T7, ->, 2171-2189
MCS: HindIII...XbaI, ->, 2198-2655
terminator: SV40 splice site and polyadenylation, ->, 2656-3352
replicon: polyoma, 3353-4188
replicon: SV40, 4189-4352
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD97
Gene name: CD97 antigen
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 p13.12-13.2
Tissue: peripheral blood mononuclear cells, PMA-stimulated
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.914
Cross references: DNA Seq. Acc.: X84700
Nucleotides 1-2914 of the insert correspond to
nucleotides 8-2921 of X84700.
Insert lengths(kb): 2.914000034332275
Tissue: peripheral blood mononuclear cells, PMA-stimulated
Gene product: CD97 antigen [CD97]
Target Gene: CD97 antigen
Insert Size (kb)
2.914
Media
ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--4.1, 2.9; SmaI--5.1, 1.9; NcoI--2.7, 1.9, 0.85 (doublet), 0.8.
Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.80, 0.96; EcoRI--2.22; EcoRV--0.79; NcoI--0.06, 2.72; NdeI--2.28; PstI--2.11, 2.65; PvuI--2.07; PvuII--0.48, 0.95; SacI--1.69; SmaI--0.35, 2.26.
References

Hamann J, et al. Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretin receptor superfamily with an unusual extracellular domain. J. Immunol. 155: 1942-1950, 1995. PubMed: 7636245

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