contains easily purifiable cassette(s) for construction lacIq - ptac promoter cassette
Comments
Restriction digests of the clone give the following sizes (kb): BglI--4.0; ClaI--4.0; XhoI--2.3, 1.65.
Helper plasmid with an XhoI promoter cassette containing the gene for lacIq and the ptac promoter.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348
