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Human rhinovirus 29
Human rhinovirus 29
規(guī)格:
價(jià)格:
編號(hào):B217345
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human rhinovirus 29
商品貨號(hào)
B217345
Classification
Picornaviridae, Enterovirus, Rhinovirus A
Agent
Human rhinovirus 29
培養(yǎng)基
Strain
5582
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
1.0 mL per vial
Storage Conditions
-70°C or colder
Images
ATCC VR-1809 Cell Micrograph
Comments
VR-1809™ has been treated for Mycoplasma orale at the ATCC using diethyl ether.  The original item number was VR-504™.
Reciprocal relationship with type 44 reported.
Effect on Host
CPE, cell rounding and cell degeneration with sloughing
Recommended Host
HeLa cells (ATCC® CCL-2™)
Growth Conditions
Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
VV Hamparian, RM Conant
Source
Human throat washings
References

Ketler A, et al. Characterization and classification of ECHO 28-rhinovirus-coryzavirus agents. Proc. Soc. Exp. Biol. Med. 110: 821-831, 1962. PubMed: 14455433

Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698

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