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lambdaACT2 - human breast cDNA library

規(guī)格:

價格:

編號:B217365

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

lambdaACT2 - human breast cDNA library

商品貨號

B217365

Designations

lambdaACT2 - human breast cDNA library

Species

Homo sapiens, human

培養(yǎng)基

Depositors

SJ Elledge

Origin

breast

Vector

Genome: human

Tissue: breast

Type of DNA: cDNA

Insert size: 0.7 – 7 kb

Number of independent recombinants: 1.0 x 10⁸

Non-recombinants (%): 5.0

Insert 5’ end:

Insert 3’ end: XhoI adapters

Titer: 9.5 x 10⁸ pfu/mL

Vector: lambdaACTII

Intact vector size (kb): 42.300

Type of vector: phage

Features:

Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII

Promoter: ADCI

Replicon: pMB1, 2 micron, lambda

Repressor gene: cI857

Terminator: ADC1

Construction: lambdaYES, pACT2 (ATCC#87006)

Vector ends: EcoRI, XhoI

Markers: ampR, LEU2

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert.

References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

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