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Hubs-5B [Hs1]
Hubs-5B [Hs1]
規格:
價格:
編號:B217401
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Hubs-5B [Hs1]
商品貨號
B217401
Designations
Hubs-5B [Hs1]
GenBank Number

M30773

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 45.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPP3R*
Genomic copy number: unique
Gene name: protein phosphatase 3 (formerly 2B), regulatory subunit
(calcineurin B)
Contains complete coding sequence?: Y
Chromosome: 2; Localization: 2
Tissue: brainstem
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M30773
Nucleotides 1-1836 of the insert correspond to
nucleotides 713-2548 of M30773.
Insert lengths(kb): 1.799999952316284
Tissue: brainstem
Gene product: protein phosphatase 3 (formerly 2B), regulatory subunit (calcineurin B) [PPP3R*]
Insert Size (kb)
1.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0, 0.35, 0.28 (doublet); BamHI--16.0, 11.0, 6.5 (doublet), 5.6; BglII--19.0, 15.0, 7.0, 2.4, 0.7;
EcoRV--5.6, 5.3, 4.5 (doublet), 3.7 (doublet), 3.3, 3.1, 2.5, 1.5, 1.4 (doublet), 1.3, 0.75, 0.6 (doublet); HindIII--21.0, 9.4, 6.0, 4.4, 3.8, 0.6; NdeI--20.0, 5.8 (doublet), 3.8, 2.4, 2.2, 1.7, 1.6, 0.55.
The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.61; EcoRI--0.27, 0.50, 1.57; EcoRV--0.53, 1.51; HincII--1.12; HindIII--0.13; NdeI--0.87; SphI--1.76.
Corresponds to nt 804-2548 of the GenBank record. It does not include the complete coding sequence (nt 759-1271).
Detects a 2.8-2.9 kb mRNA in rabbit brain, and a slightly smaller transcript in murine muscle and kidney.
References

Guerini D, et al. Isolation and sequence of a cDNA clone for human calcineurin B, the calcium-binding subunit of the Ca(2+)/calmodulin-stimulated protein phosphatase. DNA 8: 675-682, 1989. PubMed: 2558868

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